FastQCFastQC Report
Sat 18 Jun 2016
SRR3137896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433911
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132970.38722611040297783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG8550.036.7671431
TACCTGG45750.029.9596772
CGACGAG3150.028.47758724
GTACCTG50500.028.4568331
TACCCGG9850.027.339852
ACCTGGG48950.025.8685863
GTACACG6450.025.7078721
CGATACG1101.1547236E-625.08884859
ACCCGGG9900.024.3961983
TGCGACG3850.023.30018222
CGCGAGT1052.2839375E-522.9981128
GCTCGAT3300.021.95274264
CGCACTA4250.021.1041558
ATGCGAC4450.020.9342221
ACGAGTC4450.020.9336126
GAGTACT70200.020.1991212
AGTACTT67350.020.13297713
GTACCGG2252.5283953E-1019.9593071
GTACTTT67700.019.77435914
ATCGACG2002.8823706E-818.9767593
Produced by FastQC (version 0.11.5)