FastQCFastQC Report
Sat 18 Jun 2016
SRR3137896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433911
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45900.13366683061966372TruSeq Adapter, Index 10 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG9200.036.3784141
TATGCCG14750.032.27770643
TACCTGG50350.030.974112
CTCGTAT14550.030.82449739
GATCTCG14550.030.82449736
TACCCGG10300.030.8184282
TCGTATG15500.030.71588140
ATCTCGT14800.030.07070237
GTACCTG56700.028.8439791
TCTCGTA15500.028.49009338
TGCCGTC16750.028.21768445
ATGCCGT17000.028.208644
CCGTCTT17800.026.74697547
ACCTGGG56500.026.381243
GCCGTCT18000.026.06645846
CGTATGC18650.025.7129341
ACCCGGG11550.025.3919963
CGTCTTC19700.024.16731848
GCTCGAT4150.022.44527464
GTATGCC21950.021.84720442
Produced by FastQC (version 0.11.5)