FastQCFastQC Report
Sat 18 Jun 2016
SRR3137894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445716
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284760.6405267452981702No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG52600.11831614974955665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG10700.030.6672231
TACCCGG12150.025.852732
TACCTGG58900.025.4340632
CCGAACG2155.456968E-1222.4637269
GTACCTG70250.022.0275571
ACCTGGG56000.021.9960943
ACCCGGG12550.021.7194463
CGTGCGC4300.021.6624210
AGTAGGC16300.021.6000062
CGACGAG5900.019.8819124
GAGTACT85300.019.65661212
TGCGACG5300.019.52884722
GTACTTT84950.019.21050314
AGTACTT86250.019.1607513
ATGCGAC5600.019.09874721
ACGATCG2951.8189894E-1218.71070360
AGAACGC13300.018.67553162
GCTCGAT5400.018.52662764
GGGCTAT30300.018.3305446
GCTATTG29350.018.2186328
Produced by FastQC (version 0.11.5)