FastQCFastQC Report
Sat 18 Jun 2016
SRR3137894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445716
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG106110.23867921387690982TruSeq Adapter, Index 1 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG53710.12081293541917658No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG48600.10931872391308846TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29300.035.08826443
TCGTATG31150.033.1151240
CTCGTAT29600.032.98398239
TCTCGTA29300.032.85109338
GATCTCG29200.032.84544836
ATCTCGT29350.032.79512837
ATGCCGT32400.031.7314144
GTACCCG11600.031.2315371
TGCCGTC32600.031.11342645
CCGTCTT32950.030.57352847
CGTATGC36300.028.60705241
CGTCTTC37700.026.9044548
GCCGTCT38100.026.62198846
TACCCGG12800.025.877562
GTATGCC41050.025.46494142
GGCATGA41150.024.8156331
TACCTGG65600.023.6159042
ATGCGAC5900.023.38925221
AGTAGGC18400.023.25232
GCTCGAT5950.023.19322464
Produced by FastQC (version 0.11.5)