FastQCFastQC Report
Sat 18 Jun 2016
SRR3137893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113536
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG437830.856217693588155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG9550.032.1931081
CGACGAG2950.026.89938724
TACCCGG10700.026.1332072
TACCTGG61400.023.5017072
GTACCTG70300.021.3260631
GAGTACT100050.020.61990712
ACCTGGG63050.020.1397693
TGCGACG4100.019.35500722
ACCCGGG13500.018.9142763
GTACTTT106300.018.79164914
AGTACTT107550.018.7657113
CGCGTAA1852.64934E-718.64739210
ACATGGG192850.017.3020923
GTACATG276950.016.8137591
TACATGG269200.016.6709272
GTACGTG6450.016.6026821
CCTGGGG46700.016.4731944
ACTTTTT121800.016.45788216
ATGCGAC5100.016.23642321
GGTCGTC7700.016.12878452
Produced by FastQC (version 0.11.5)