FastQCFastQC Report
Sat 18 Jun 2016
SRR3137893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113536
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG109070.21329663074631722RNA PCR Primer, Index 26 (100% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36750.033.6074943
CTCGTAT33400.032.433839
GTACCCG11500.031.5042971
TCTCGTA33700.031.32609238
ATGCCGT38900.031.21788444
TGCCGTC38900.030.41969945
ATCTCGT37550.029.6761337
TCGTATG36950.029.59780540
GCCGTCT39050.029.24269346
CCGTCTT40300.028.849347
CATCTCG40850.028.12333736
CGTCTTC41150.026.99581548
CGTATGC45900.026.6075741
TGCGACG3050.026.01587522
ATCGCGT7600.025.87473537
TACCCGG13500.025.3034532
GTATGCC50050.024.81493242
CATCGCG9400.024.22315836
TACCTGG60450.024.0305752
GAGCATC62100.023.38842233
Produced by FastQC (version 0.11.5)