FastQCFastQC Report
Sat 18 Jun 2016
SRR3137892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221666
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184800.5736162594136077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG5350.031.6430931
TACCTGG47950.029.8771062
ACCTGGG44500.027.6824913
GTACCTG54150.027.3713041
TACCCGG5850.025.9642542
CCTGGGG31150.024.0327954
GAGTACT82100.021.85158712
CCGGCGT5400.021.72038769
TATCACG1756.170012E-921.6986982
AGTACTT80250.021.62515613
GTACTTT80050.021.33504914
CTGGGGG19050.020.825015
ACCCGCG5750.020.39827558
CGTCCCG1056.025875E-419.71445512
CGCAACG900.005739416519.16504742
GTACACG7650.018.968131
ACTTTTT90600.018.92683416
TAAATCG3100.018.91775562
ATCGACG1651.827304E-618.8215563
ACCCGGG7250.018.5619493
Produced by FastQC (version 0.11.5)