FastQCFastQC Report
Sat 18 Jun 2016
SRR3137892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221666
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG75560.2345370376693301RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.10224523585002293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18900.035.4119939
TATGCCG19500.034.8531543
TCTCGTA20000.033.29183238
ATGCCGT20650.032.74510644
TGCCGTC21100.031.88324745
CCGTCTT21350.031.3483247
TACCTGG53200.030.290282
TCGTATG23000.029.24941440
GTACCCG7600.029.0579131
ACCTGGG48750.028.8790953
AATCTCG23200.028.25374636
GCCGTCT23900.028.00362446
GTACCTG59450.027.8023621
ATCTCGT24250.027.45718237
TACCCGG7800.027.428062
CCTGGGG34000.024.8652024
CGTCTTC27500.024.33769648
TAGGGCG3300.024.04497331
GTATGCC29100.023.82941842
GAAATCT27450.023.75362434
Produced by FastQC (version 0.11.5)