FastQCFastQC Report
Sat 18 Jun 2016
SRR3137891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448850
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG724811.330207291446819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7900.033.19220424
TGCGACG9050.029.35592322
ATCGTCA10650.026.23718810
ATGCGAC11250.024.842221
GTACCCG11650.023.4313321
GCTCGAT9850.022.41411664
GTACTAG14600.022.2469651
CTCGATA10350.021.33130865
TACCTGG67200.021.3229562
CGCACTA12800.020.4824358
GTCGTCT11150.020.41958653
GCGACGA13700.019.64371523
GTACACG10800.019.5164971
GAGTACT61100.019.36754612
CCGAACG2505.0931703E-1119.31816769
GTACCTG77500.019.305541
ACGAGTC13600.019.02672226
GCGCACT13600.019.0239357
CGTCATC15450.018.98069612
ACCTGGG69800.018.6374863
Produced by FastQC (version 0.11.5)