FastQCFastQC Report
Sat 18 Jun 2016
SRR3137891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448850
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG294980.5413619387577195TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG125880.23102122466208463TruSeq Adapter, Index 1 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG71480.1311836442552098RNA PCR Primer, Index 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG77550.035.67891743
CTCGTAT76300.034.45446839
GATCTCG76100.034.27269436
ATCTCGT76950.033.89411537
TCGTATG80650.033.7938640
TCTCGTA76950.033.75992638
ATGCCGT82900.033.41797644
TGCCGTC83100.033.1296645
CGTATGC85450.032.3396741
CCGTCTT86150.032.03655647
GCCGTCT87100.031.48908846
CGTCTTC87750.031.33446748
GTATGCC91700.030.36122742
GGATCTC87000.029.9393835
CGACGAG9450.029.20581424
TGCGACG9550.028.90025922
GTACCCG12300.028.332651
ATCGTCA10250.027.60101110
AAGGGGG90400.027.55344669
TGCTTGA99500.027.35653355
Produced by FastQC (version 0.11.5)