FastQCFastQC Report
Sat 18 Jun 2016
SRR3137890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3637290
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128380.3529550846921746No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG76540.21043139260273447No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT61750.1697692512832356No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA58250.16014670262750563No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT51640.14197383216625564No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC49370.1357329220381108No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTA46200.1270176422556354No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG36900.10144915582755293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG9750.038.266141
CGACGAG8500.036.5301524
ATCGTCA9300.032.64447810
ACCTGGG54150.031.985793
TACCTGG62700.031.6030582
ATGCGAC10700.029.98659321
TGCGACG10700.029.01928322
TGGTCGT8100.028.9611651
CTGGGGG26100.028.9464745
TAGGCAT12650.028.6345835
GCTCGAT8200.028.60797964
GTACCTG72250.028.0191691
ACCCGGG12200.027.71523
CCTGGGG35900.026.9071454
AGTAGGC12450.026.8959662
ACGAGTC11900.025.80304526
GTCGTCT9250.025.73353853
GCATCGT12250.025.0640958
GTCCTAC11850.025.0381430
GTCAGCG10000.024.83845543