FastQCFastQC Report
Sat 18 Jun 2016
SRR3137890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3637290
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG80000.21994396927382748No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT69180.19019654742954234No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA59210.16278603025879157No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT57460.1579747559309266No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC51690.14211129714705178No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTA46620.12817234809432296No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG44600.12261876287015883No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42120.11580049982267018TruSeq Adapter, Index 8 (96% over 29bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT38310.10532566828600413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG10400.036.8242151
GCTCGAT11100.036.67524364
TGGTCGT11400.034.80270451
ATCGTCA10600.034.5000710
CGACGAG10600.033.52319324
TGCGACG10600.032.87225722
CTCGATA12450.032.144265
ACCTGGG55950.031.8203833
GTCAGCG13100.031.60268243
TACCTGG63900.030.9923762
GTCGTCT13050.030.66630753
GCTCGTC13400.030.637739
TCGATAA13100.030.28590466
ATCTCGT12550.030.23868837
TAGGCAT13650.030.0798785
TATGCCG13200.029.79510543
GCGCTGG13850.029.64265847
CGATAAT13700.029.21133467
ATGCGAC12650.029.18147721
GTCTACC14050.028.97474956
Produced by FastQC (version 0.11.5)