FastQCFastQC Report
Sat 18 Jun 2016
SRR3137889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130472
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247490.5991809168540544No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG56150.13594088036427798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50050.026.6932532
GTACCCG6900.025.033761
GTACCTG54950.025.0219141
ACGTGCG2100.024.6411849
ACCTGGG52450.022.6942483
GCGCGAT2450.022.53208413
GCTCGAT4200.022.17706564
GAGTACT95650.021.96639312
TTAGTCG4300.021.66158139
TAGTCGG4350.021.41233840
GTACTTT95700.021.34308614
CGTGCGC3800.020.88016310
AGTACTT101350.020.52773313
TACCCGG8050.019.2979552
ACTTTTT106300.019.2474916
CGCGGTA1852.6490488E-718.64738343
ATGCGAC4650.018.54959721
CCTGGGG39750.018.4859854
ATAACGG11300.018.3173463
TACTTTT114900.017.8368915
Produced by FastQC (version 0.11.5)