FastQCFastQC Report
Sat 18 Jun 2016
SRR3137889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130472
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG88350.21389807266578736TruSeq Adapter, Index 8 (96% over 29bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG56750.13739349885436822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24150.030.57125943
CTCGTAT23550.030.47116739
TCTCGTA25150.028.80699538
CCGCGAA1202.6902853E-928.74949345
TCGTATG25600.028.16585740
ATCTCGT26200.027.7838637
TGCGACG4550.027.29621922
ATGCCGT27400.026.94510344
TGCCGTC27300.026.91710545
AATCTCG27150.026.81168236
GTACCCG8550.026.6359441
CCGTCTT28250.026.50041647
TACCTGG52250.025.6893752
TAGGGCG2800.024.64242231
GCCGTCT30050.024.22419546
GTACCTG58100.023.9342161
ATGCGAC5450.023.42160421
GAATCTC31750.023.3617935
CGTACGA3550.023.3235360
CGTCTTC31400.023.2925848
Produced by FastQC (version 0.11.5)