FastQCFastQC Report
Sat 18 Jun 2016
SRR3137888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5065098
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220160.4346608890884243No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG65300.1289214937203584No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA57420.11336404547355253No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT54470.1075398738583143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG6800.026.9210991
TACCTGG62100.026.346892
GCTCGAT6900.024.4978564
GTACCTG73450.023.0425281
CGACGAG6500.022.8251324
ACCTGGG59050.022.788053
GTACTAG10200.022.010961
GTACTTT86850.021.0945414
GAGTACT88550.021.03960812
AGTACTT89400.020.68540413
ATCGTCA7400.020.51191710
ATGCGAC9250.020.14255121
CGTGCGC7800.019.90229810
GCGCGTA2102.3537723E-919.71508613
TCTCGCG1258.747117E-519.32040432
TGGTCGT8750.018.9242451
ACTTTTT97700.018.71732316
GCGCGAT3700.018.64940613
CTCGATA9350.018.07862965
CCTGGGG47050.017.8172444
Produced by FastQC (version 0.11.5)