FastQCFastQC Report
Sat 18 Jun 2016
SRR3137888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5065098
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG76250.15054002903793767TruSeq Adapter, Index 8 (96% over 29bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG67550.13336365851164184No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT61120.12066893868588524No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT55560.10969185591275825No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA54960.10850727863508268No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC52810.10426254339007852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21050.030.32023443
ATCTCGT22700.027.66012837
GTACCCG6950.027.3068921
TCTCGTA23300.027.24425138
CTCGTAT23900.026.41594539
TCGTATG24100.026.33987640
TACCTGG67000.025.544732
TGCCGTC25300.025.36302845
AATCTCG24950.025.1657336
GCTCGAT6600.025.09030564
ATGCCGT26400.024.30647544
CCGTCTT25950.024.06324247
CGTATGC27700.023.29028341
GTACCTG75750.023.18621
CGATATA2550.022.99944762
ACCTGGG62900.022.7118593
AGTACTT131800.021.28080613
CGTCTTC29700.021.25727348
GAGTACT132500.021.01236312
GCCGTCT29900.020.99949546
Produced by FastQC (version 0.11.5)