FastQCFastQC Report
Sat 18 Jun 2016
SRR3137887_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137887_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4844023
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365320.7541665264595152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT12000.030.7602264
GTACCCG15600.030.1169721
TGGTCGT12250.029.85085151
CTCGATA13000.027.59795665
TACCTGG73500.027.1943452
TCGTCTA13950.026.46040754
GCTCGTC14100.025.93505339
GTCGTCT14900.025.23638553
CTGGTCG15100.024.6736750
TGCGACG10150.024.47463222
ATGCGAC11150.024.4456721
GTACCTG88050.023.7368471
ACCTGGG77600.022.8543593
CGTCTAC16500.022.37106955
ATCGTCA10500.022.34143410
GTACTAG14600.022.0052911
CCGGCGT8000.021.9921269
CGTCATT17000.021.91874126
CATTGCG16950.021.57562829
GTCGTCA17800.021.51511824
Produced by FastQC (version 0.11.5)