FastQCFastQC Report
Sat 18 Jun 2016
SRR3137887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4844023
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG131110.2706634547358673TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG58660.12109769090691766TruSeq Adapter, Index 8 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT11550.033.7522164
TATGCCG38300.033.4180343
TCGTATG39400.032.13479640
CTCGATA12150.031.51754865
CTCGTAT39450.031.2195739
TGCGACG9800.030.97895622
TCGATAA12600.030.66572266
TCTCGTA40950.030.24449238
CGTATGC42250.030.21208641
CGATAAT13250.030.20284367
TGGTCGT12850.030.06911351
TCGTCTA13350.029.97660454
AATCTCG42950.029.39839736
GCTCGTC13650.029.06503739
ATGCGAC11350.028.57210221
ATGCCGT44950.028.55084644
TGCCGTC45400.027.96389845
CTGGTCG14600.027.88270250
TACCTGG77850.027.792732
GTACCCG16400.027.5644761
Produced by FastQC (version 0.11.5)