FastQCFastQC Report
Sat 18 Jun 2016
SRR3137886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688804
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158500.4296785624825824No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG48350.13107229335036505No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT37410.10141498436891741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG10400.038.1915471
CGACGAG5300.032.54810324
TACCCGG11850.030.29292
TACCTGG55400.029.40752
ATGCGAC6450.028.88533221
ACCCGGG11750.027.8955523
GTACCTG62200.027.4863821
TGCGACG6450.026.74567822
GCTCGAT5350.026.4378664
ACCTGGG56700.025.070523
ATCGTCA7500.024.37904510
GCGACGA7650.023.45228823
TGGTCGT6250.023.18278151
CGCACTA7350.022.52942858
TCGTCTA6900.022.49881454
GTCGTCT6550.022.12097253
CTCGATA6600.021.9533965
GCGCACT7600.021.78832857
GAGTACT84050.021.34418112
GTACACG7950.021.2878461
Produced by FastQC (version 0.11.5)