FastQCFastQC Report
Sat 18 Jun 2016
SRR3137886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688804
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG56230.15243423071542972TruSeq Adapter, Index 3 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG49820.13505732481313729No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT40020.10849044839465583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG10800.034.1843571
TATGCCG16200.033.64732743
TGCGACG6000.031.62465922
CTCGTAT16850.030.7114239
ATCGTCA6700.030.38150410
GTCGTCT6300.030.11872353
TACCTGG55750.030.0786822
TCGTCTA6550.029.49586754
TCGTATG18400.029.24928940
TCTCGTA17600.029.01065838
ATGCCGT19400.028.27508544
GTACCTG62000.028.1039331
CGTATGC19400.027.91942441
AATCTCG18300.027.9009636
CCGTCTT19600.027.81054747
CGACGAG7250.027.59970324
TGCCGTC19800.027.52963345
ATCTCGT18550.027.52493537
GCTCGAT6550.027.38864764
GCGACGA7450.027.32185223
Produced by FastQC (version 0.11.5)