FastQCFastQC Report
Sat 18 Jun 2016
SRR3137885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4551226
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG99210.21798521980670704No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG93100.20456026573938538TruSeq Adapter, Index 3 (96% over 28bp)
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT78570.17263480213902804No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA73550.16160480714427278No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT72020.15824307560204656No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC65960.14492798204264082No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTA55660.12229671741196767No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA53210.11691355252408911No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT46420.10199449554911139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25000.034.91341843
ATCTCGT26050.031.38719237
TCGTATG27650.031.31774140
ATGCGAC10350.030.33249521
ATGCCGT29100.029.87579244
TGCCGTC28600.029.79495845
TGCGACG9800.029.5706122
CTCGTAT28500.029.29424939
ATCGTCA10650.028.83018710
TCTCGTA28900.028.29191638
AATCTCG29250.028.1892736
CGTATGC31550.027.44645141
ACGTCGA2900.027.36131365
CCGTCTT31450.026.87554447
TGGTCGT9000.026.83376951
CGTCTTC32700.026.27077948
GCCGTCT32850.025.73016446
GAATCTC32200.025.60671435
GCTCGAT9450.025.55484864
CGACGAG11800.025.14364824
Produced by FastQC (version 0.11.5)