FastQCFastQC Report
Sat 18 Jun 2016
SRR3137884_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137884_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303555
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23900.18334477639992175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT200.007361242551.7464661
GTACCCG8350.051.29891
TACCCGG8900.046.150842
TACCTGG50200.043.935932
CCCGGGA4800.043.1237074
ACCCGGG7650.042.8467833
CCTGGGG12700.042.1050344
ACCTGGG40350.042.070493
CGGGAAG5250.042.0542686
CTGGGGG8950.040.0866435
CCTGGTA9600.038.8113374
ACACGGG4450.038.767343
GTACCTG58050.038.382241
CCCGGTA901.2369128E-1038.3321844
CCGGGAA4950.038.330715
CCGGTAA901.2369128E-1038.330715
GCTCGAT1350.038.3307164
ACCTGGT10050.037.7644043
CCTGGGA25550.035.9167334
GTACCGT1550.035.658341
Produced by FastQC (version 0.11.5)