FastQCFastQC Report
Sat 18 Jun 2016
SRR3137884_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137884_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303555
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG9450.048.192651
TACCCGG10800.040.8907282
ACCCGGG9700.040.5482183
TACCTGG56100.040.221062
CGGTAAG1550.040.0678566
ACCTGGG42500.039.940623
TATGCCG1850.039.16242243
CCCGGGA6000.036.801664
CGAAGGC754.022513E-836.79883669
CCTGGGG14550.036.5171054
GTACCTG65250.034.9511
CGATCGA709.739979E-734.4989165
CCGGGAA5500.033.8755535
GCTCGAT1253.6379788E-1233.1189564
CTGGGGG9400.031.9335145
CCTGGGA27400.031.8572754
ACCTGGT13300.031.648043
GCGCCTA1207.8216544E-1131.62521243
CCGTCTT2300.031.5002147
CGGGAAG6500.031.3179936
Produced by FastQC (version 0.11.5)