FastQCFastQC Report
Sat 18 Jun 2016
SRR3137883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895864
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159340.40899785002761907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG11950.041.048281
TACCCGG14200.033.5521052
TACCTGG68000.031.935282
ACCCGGG14300.031.6075653
GTACCTG77600.029.0687261
ACCTGGG69350.028.6073173
ACGTGCG1850.027.9713889
TACACGG10600.025.730622
CGTGCGC4350.024.58481410
GCGCGAT2250.023.00105913
GTACACG12600.022.4811151
CCTGGGG46550.022.3067194
ACACGGG11400.021.4886573
CGCCGAA5150.021.43512755
ATGCGAC5050.021.18055221
TGCGCGA2650.020.83007812
CCGAACG3350.020.59531869
CGACGAG5200.020.56851824
GAGTACT88100.020.44143312
GCTCGAT4600.020.24833364
Produced by FastQC (version 0.11.5)