FastQCFastQC Report
Sat 18 Jun 2016
SRR3137883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895864
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG61050.15670464882757715RNA PCR Primer, Index 33 (96% over 29bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG39730.10197994591186962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG12550.036.2915651
TACCTGG70700.035.334152
GTACCTG79800.031.823681
TACCCGG13950.031.6600232
ACCTGGG73700.030.8995323
TATGCCG15100.030.61554743
CTCGTAT15700.028.56655339
TCTCGTA15550.028.39839238
CCTGGGG47100.025.787454
ACCCGGG15550.025.7396773
TCGTATG18000.025.6830440
ATGCCGT18250.025.52025644
ATCTCGT17650.025.21501437
CCGTCTT18200.025.21125247
TGCCGTC18200.025.21125245
AATCTCG17950.024.60139336
GCTCGAT4650.024.48359364
CTCGATA4950.022.9997465
GAGTACT128150.022.47926112
TGGTCGT5300.022.13182451
Produced by FastQC (version 0.11.5)