FastQCFastQC Report
Sat 18 Jun 2016
SRR3137882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5812199
Sequences flagged as poor quality0
Sequence length75
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161160.2772788750006667No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA76570.1317401554902026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG9200.035.6700971
CTCGTGC14750.035.0826266
TCGTGCA12200.035.0634567
TATGCCG7450.034.7294450
AGTAGGC37600.033.78922
GACTAAC27550.032.68222847
AGTACGC38300.032.5406652
TACCTGG62900.032.108732
CGATCCG4300.032.0910866
ACTAACC48450.031.18696448
CGCAGTG40350.031.1207926
TAGGCAG40600.031.0146664
CTAGTGC10250.030.96397866
GTAACCC20650.030.90611849
ACCTGGG52400.030.7526843
TAAACCC52250.030.63546439
TGGCTAA79550.030.61662356
GTACGCA40850.030.493873
CGCATGG19150.030.44463243
GACTCAA37100.030.40651559
Produced by FastQC (version 0.11.5)