FastQCFastQC Report
Sat 18 Jun 2016
SRR3137882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5812199
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA156920.269983873573496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG9000.041.023551
CGAGTAC17650.038.89751469
AGGCTAA45050.037.9839769
ACGACGA25150.035.52836665
AGCGAGA13800.033.99955769
TACCTGG63950.032.1586382
TATGCCG16400.031.76483243
CAGGCTA69950.031.02220568
ACCTGGG51150.030.7617573
GTACCTG70450.029.7793031
CAACGAC34150.029.09479163
GCCGAAT3450.028.99962267
CCTGGGG30650.028.9330734
AACGACG33650.028.80943364
CGACGAA12000.028.74962666
AGAGTCG13450.028.7282569
ATGCCGT18050.028.47885744
GTAAGCG36650.028.1453749
TGCCGTC18650.028.11735545
TACCCGG12900.028.0861052
Produced by FastQC (version 0.11.5)