FastQCFastQC Report
Sat 18 Jun 2016
SRR3137881_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137881_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826735
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111720.39522629464735815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG12150.047.4878121
TACCCGG12150.045.461872
TACCTGG71850.040.312412
ACCCGGG13300.038.6572273
ACCTGGG70650.038.437813
GTACCTG81850.036.0057831
CCTGGGG35100.034.595854
GTACACG9900.033.1535721
ACACGGG8450.033.0768663
TACACGG10250.029.9757272
CTGGGGG21000.029.5693445
GCTCGAT2500.028.97795764
CCTGGGA38200.025.828024
TACCGGG1359.373252E-925.57232
CACGGGG6050.025.089144
ATGCGAC3050.024.88657221
GTACCGG1701.6552804E-1024.3878921
CCCGGGA6250.023.1823644
TGCGACG3000.023.00081822
ATCGTCA3450.022.99878510
Produced by FastQC (version 0.11.5)