FastQCFastQC Report
Sat 18 Jun 2016
SRR3137881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826735
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41660.1473785126656726RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.10375928412107963No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA28600.1011767993816187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG14000.044.6083641
TACCCGG14700.041.0758362
TACCTGG72650.040.084142
ACCTGGG71950.037.980453
GTACCTG81500.036.197141
ACCCGGG15650.036.1572383
CCTGGGG34750.036.0433584
TATGCCG11500.033.5994543
CTGGGGG22550.030.7560565
CGACGTA450.004624190730.66616659
CTCGTAT12400.030.60433639
TCTCGTA12300.030.01170538
TGCCGTC13000.029.7225945
CCGTCTT13100.029.23234747
ATCTCGT12800.029.10889837
ATGCCGT13500.028.62175444
AATCTCG12750.028.14071336
GCCGTCT14050.027.50132846
TCGTATG13800.027.49954840
ACACGGG10100.026.9880473
Produced by FastQC (version 0.11.5)