FastQCFastQC Report
Sat 18 Jun 2016
SRR3137880_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137880_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606145
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192010.7367586991514287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG31850.025.3612562
GTACCTG35900.023.1870481
GTACCCG5500.022.6080271
ACCTGGG35950.021.6898783
ATAGTCG800.002898980921.5643320
TACCCGG5600.020.9582582
ATGCGAC3000.020.70175621
CGCACTA2900.020.22274858
GAGTACT56050.019.88113612
GTACTTT53900.019.7795914
CGCTAGT1751.4735087E-719.7155815
AGTACTT56000.019.65396913
TCGAGGC2951.8189894E-1218.7105848
ACTTTTT59700.018.37844316
CGCGTAA1150.001111248717.99876465
GTATCAA179950.017.9466171
GTACTAG5600.017.8868061
CCTGGGG26950.017.7928414
GCGACGA3600.017.25146523
TAGTGCG3600.017.25146524
Produced by FastQC (version 0.11.5)