FastQCFastQC Report
Sat 18 Jun 2016
SRR3137880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606145
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG73220.2809513668656195TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31270.1199856493019383TruSeq Adapter, Index 10 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG26800.10283387915868074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19950.033.72039843
CTCGTAT19200.032.5220839
TCGTATG21200.031.89489640
TCTCGTA19300.031.45982738
TGCCGTC22400.030.1862445
ATGCCGT22650.029.85306244
CCGTCTT22900.029.67780147
TACCTGG33100.028.4611422
CGTATGC23800.028.4105841
ATCTCGT21800.027.85204937
GCCGTCT24850.027.34895946
GATCTCG22100.027.16176436
GTACCCG6250.026.5020141
CGTCTTC25800.026.4756448
GTACCTG38600.024.9421881
CCGAGGC28400.024.41623528
GTATGCC28150.024.14286642
ACCTGGG36400.024.079643
TACCCGG6250.023.7413882
ACCGAGG29650.023.27052927
Produced by FastQC (version 0.11.5)