FastQCFastQC Report
Sat 18 Jun 2016
SRR3137879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3738341
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184510.49356118128335535No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG39840.10657133739270977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG12950.041.8847271
TACCCGG14600.036.4134832
TACCTGG70100.032.3550222
ACCCGGG15000.031.7427023
ACCTGGG70850.029.4626373
GTACCTG80950.028.5092241
TACACGG10150.025.508772
CCTGGGG41100.025.4320494
GAGTACT73700.021.86044312
GTACTTT74000.021.3070414
AGTACTT76600.020.7637113
AACGACA6900.020.4981865
TAGGGCG2750.020.07309231
TAGTCGG2603.6379788E-1219.90234640
ACACGGG10950.019.8510063
CTGGGGG27200.019.7850075
GTACACG13800.019.527261
ACTTTTT81850.019.47560516
ACGGGGG4900.018.3044995
AAACGAC11750.018.20263964
Produced by FastQC (version 0.11.5)