FastQCFastQC Report
Sat 18 Jun 2016
SRR3137879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3738341
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG73290.19604953106204062RNA PCR Primer, Index 39 (96% over 30bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG40350.10793557891053812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.10643758822429521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG14500.037.598951
TACCTGG73750.033.5462342
TACCCGG16250.032.7004662
TATGCCG20300.031.78062643
TCGTATG21250.030.68455140
TCTCGTA21250.030.35984638
CTCGTAT21550.030.09729839
ACCTGGG76750.029.9870723
ATCTCGT21500.029.84596437
CCGTCTT22000.029.79490747
GTACCTG84150.029.7693351
TGCCGTC22300.028.9299645
ATGCCGT22500.028.82651944
GCTCGAT3750.027.59949164
ACCCGGG17100.027.241083
CGTATGC24450.026.95078541
TATCACG2100.026.28982
CATCTCG26100.025.1144836
CGTCTTC26000.024.94569448
CCTGGGG43550.024.8787234
Produced by FastQC (version 0.11.5)