FastQCFastQC Report
Sat 18 Jun 2016
SRR3137878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4225285
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173740.4111911977535243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGC24100.029.0773642
GTACCCG7650.028.4464321
GTAGGCA26150.027.7084873
GCGCGAT2050.026.92777613
AGTACGC13150.025.9887542
TACCTGG44100.025.9881552
TAGGCAG26200.025.9396044
ACCTGGG44500.024.7340893
TGTCGCG700.0013339824.64430623
TATACCG2950.024.5575715
GCTCGAT4250.024.35120464
GTCGTCT3850.024.19307953
TGGTCGT4000.024.14827751
GTACGCA13600.024.1018243
TGCGACG3500.023.65853522
GAGTACG17100.023.6340641
CGCAGTG14650.023.312336
GTACCTG50700.023.3005831
GTCGCGC750.001993355623.00108124
CGCCGGA2300.022.50105514
Produced by FastQC (version 0.11.5)