FastQCFastQC Report
Sat 18 Jun 2016
SRR3137878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4225285
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA89030.21070768007365182No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG43340.10257296253388824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG6650.031.6502321
TACCTGG46850.026.8077562
GCTCGAT4550.025.77981264
AGTAGGC27100.025.4641632
TATGCCG13400.024.97348443
TGCGACG4950.024.3935522
CGAGTAA10550.024.19866869
GTAGGCA30300.024.0274963
GTACCTG54050.023.8750671
ACCTGGG49050.023.4950373
TGGTCGT5300.023.4335951
GCTCGTC5350.023.21485739
CAACGAC4450.022.48278863
CTCGATA5400.022.36075665
ATCGTCA5650.021.98247110
ACGAGTA6300.021.90441368
CTCGTAT15000.021.84991339
ATCTCGT15150.021.63357437
TCTCGTA14500.021.41370438
TACCCGG9350.021.4035152
Produced by FastQC (version 0.11.5)