FastQCFastQC Report
Sat 18 Jun 2016
SRR3137877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552305
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203640.5732615864910249No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG57860.162880158094533No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT45160.1271287234626531No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA44020.12391953956656311No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT40940.11524911289993399No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC38950.1096471164497418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG306.3156715E-445.99641459
GCTCGAT4850.034.85295564
CGACGAG3900.033.61702324
GTACCCG4200.032.8971821
TGGTCGT6200.028.3768251
ATCGTCA5050.028.00850710
TGCGACG4750.026.87499422
ATGCGAC5050.026.64486321
CGCATGG5000.026.21795543
GTCGTCT7050.024.95550253
GTCAGCG7350.024.87561443
GCTCGTC7400.024.24169339
CCCCGTA2450.023.93994325
GTACTAG7700.023.775691
TACCTGG36550.023.7067072
CGTCATT7350.023.47053326
GCCGAGG9400.023.12053953
CTCGTGC5100.022.99820966
CATTGCG7950.022.56650429
TCGTGCA4950.022.30129267
Produced by FastQC (version 0.11.5)