FastQCFastQC Report
Sat 18 Jun 2016
SRR3137876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374205
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335740.7675451882113435No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG49230.11254616553179378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG10200.032.852971
GCTCGAT5700.026.62900764
TACCCGG12200.026.0332242
TACCTGG59550.025.7975352
CGACGAG5050.025.27969724
GTACCTG67900.023.6584471
ACCCGGG12150.023.5712833
TGGTCGT6150.022.99777851
ACCTGGG60750.022.8329073
TGCGACG5350.022.57255722
GAGTACT101500.022.19545212
GTCAGCG7050.022.0191543
GCTCGTC6800.021.81456839
ATGCGAC5700.021.79185321
CTGTGCG9500.021.7876179
TGCGCGA3650.021.73961412
CGCGATA3700.021.44730814
CCTGGGG38900.021.2837774
GCGCGAT3750.021.16061813
AGTACTT105200.020.75963613
Produced by FastQC (version 0.11.5)