FastQCFastQC Report
Sat 18 Jun 2016
SRR3137876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374205
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG138650.3169718840337844RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG58960.13479020759200813RNA PCR Primer, Index 17 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG53940.12331383645713906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33600.033.78077743
CTCGTAT31700.033.19354239
TCTCGTA32400.032.68898838
TCGTATG34850.032.2721540
AATCTCG33900.031.3443436
GTACCCG9700.030.9471151
ATCTCGT34800.030.63284337
CCGTCTT37400.030.44075247
ATGCCGT37600.030.27883344
TGCCGTC38000.030.050945
CGTCTTC39200.029.04296348
CGTATGC39000.029.0149841
GCCGTCT39800.028.51844646
GTATGCC44050.025.8452742
CGACGAG4950.025.09027324
TACCTGG62050.024.7451932
TGCTTGA49950.023.1380855
GTACCTG69500.022.6884081
ACCTGGG61750.022.630323
TACCCGG12950.022.3811382
Produced by FastQC (version 0.11.5)