FastQCFastQC Report
Sat 18 Jun 2016
SRR3137875_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137875_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228431
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG755723.391264975222477No Hit
GTACTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAA31960.14341929366446618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT1650.037.63489552
GTACCCG7200.036.452871
TGGTCGT4900.034.49865751
CCGTATC1800.034.49865355
GCTCGAT5200.033.17178364
GTCGTCT5650.031.75097353
ACCCGGG7600.030.8679073
CTCGATA5600.030.80236865
ACGCTAA450.004624495730.66546865
ACTACCG1700.030.4399960
TACCCGG8100.030.2517762
GTCTACC6150.029.73054756
CGACTTA1301.9826984E-1029.1911767
GCCGTAT2250.029.13219854
TACCTGG45000.028.9138852
GTTTGCG854.431946E-628.41065655
TGGTCGC2200.028.22617149
TACGTAC500.007737923427.5989234
ACCTGGG42300.027.2406833
TGCGACG2800.027.1079122
Produced by FastQC (version 0.11.5)