SRR3137875_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3137875_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2228431
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

$Per base sequence content$

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	28284	1.2692338241569967	RNA PCR Primer, Index 33 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	15206	0.6823635104699226	RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	9971	0.44744486142940926	RNA PCR Primer, Index 33 (96% over 29bp)
GTACTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAA	6763	0.30348707229436317	No Hit
GCTTAAGTACTCTGCGTTGATACCACTGCTTAAGTACTCTGCGTTGATACCACTGCTTAAGTACTCTGCGTTGAT	3640	0.16334362607592517	No Hit
CTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAACGC	2824	0.126725934076487	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG	2396	0.1075195956258013	RNA PCR Primer, Index 15 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	2372	0.10644260468464135	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG	2244	0.10069865299845496	RNA PCR Primer, Index 33 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG	2229	0.10002553366023001	RNA PCR Primer, Index 15 (96% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCTCGAT	540	35.138203	64
TGGTCGT	555	34.190056	51
GTCGTCT	590	32.16183	53
CTCGATA	590	32.16039	65
ATCTCGT	7310	31.62045	37
TATGCCG	7960	31.552145	43
TCGTATG	8040	31.281107	40
TCTCGTA	7415	31.265743	38
CGTATGC	8075	31.017351	41
CCGTCTT	8065	31.013037	47
CTCGTAT	7580	30.994776	39
AATCTCG	7520	30.691555	36
ATGCCGT	8225	30.49363	44
CGTCTTC	8255	30.341702	48
GTATGCC	8285	30.314434	42
TGCCGTC	8240	30.270647	45
CTTGAAA	8305	30.158354	57
GTCTACC	640	29.648525	56
GCTCGTC	640	29.64786	39
TACCTGG	4660	29.320312	2