FastQCFastQC Report
Sat 18 Jun 2016
SRR3137874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766407
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198070.5258858110660903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG10700.035.8420641
TACCTGG58750.033.3227122
TACCCGG11600.031.2533452
GTACCTG68750.029.6505761
ACCTGGG63550.029.1596053
ACCCGGG10950.028.6780853
GCTCGAT4100.024.40081864
CCTGGGG41500.023.9408974
GCTCGTC4850.022.76197839
GTACACG10400.022.5906831
TGGTCGT4800.020.84236751
GAGTACT87700.020.7309212
GTACTTT86700.020.37370314
TACACGG10900.020.273032
AGTACTT89000.020.19579913
ACACGGG10150.019.7189983
GGTCGTC7300.019.37542752
CTGGGGG28050.018.9399195
CTCGATA4750.018.88295665
GCGCGAT2053.830064E-818.5119513
Produced by FastQC (version 0.11.5)