FastQCFastQC Report
Sat 18 Jun 2016
SRR3137874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766407
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG76870.2040937158411186RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39090.10378591586092528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG13700.036.014931
TACCTGG61000.032.8069082
TATGCCG18650.032.7427743
CTCGTAT19550.031.2350239
TACCCGG14550.030.8281542
TCTCGTA19700.030.29668638
GCTCGAT3900.030.07625864
TGCCGTC20650.029.7386345
GTACCTG68900.029.5460831
ATGCCGT20950.029.14809844
ACCTGGG65650.028.6440663
GCCGTCT21850.028.10538746
ACCCGGG14900.028.020253
CCGTCTT22350.027.78535747
TCGTATG22550.027.23257440
ATCTCGT23100.025.98643937
CGTCTTC25000.024.5641148
CGTATGC25000.024.56378441
TGCGACG3600.023.95780222
GCGACGA3750.023.9194723
Produced by FastQC (version 0.11.5)