FastQCFastQC Report
Sat 18 Jun 2016
SRR3137873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866921
Sequences flagged as poor quality0
Sequence length75
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172600.3546390007152366No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG71670.14725942746964663No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT62270.12794536833451786No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA56690.1164802140819627No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC51790.10641224708599134No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT50520.1038027944156069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA5650.042.13025710
GTACCCG12150.040.0923421
GCTCGAT6100.039.0222964
TAGTGCA4100.038.70503267
TGGTCGT6250.038.63772251
GTAGGCA40150.037.2133263
AGTAGGC41250.036.3214152
CTAGTGC5250.036.14072866
TAGGCAG41750.035.8620954
TATGCCG4000.035.36041650
ACTAGTG4150.034.913665
CTCGTGC6500.033.96722866
ACCTGGG57400.033.123473
TACCTGG66000.032.848282
GCGCTGG7900.032.75123647
GTCAGCG7600.032.68228543
CCTGGGG21450.032.4882624
CGGTCCG751.671071E-632.19909328
CGACATC4850.032.00842366
TAGGCAT9500.031.9563395