FastQCFastQC Report
Sat 18 Jun 2016
SRR3137873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866921
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA93030.19114754482351368No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG74600.1532796607958091No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT69310.14241036581444408No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG64370.1322602113327913TruSeq Adapter, Index 1 (96% over 28bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA58160.11950060418075412No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC56680.11645966721054235No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT56290.1156583392251487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA6050.045.62060510
TGGTCGT7700.040.77257551
GCTCGAT7600.040.4007564
TGCGCAA350.001348272139.42842568
GTACCCG14300.037.398041
GCTCGTC8600.036.50530239
ATCTCGT15300.036.07792337
GTCGTCT8800.035.67637353
TATGCCG17000.035.5142143
ACGACGA17700.035.08425565
GCATTAC32650.034.869769
GCGCTGG9500.034.13671547
GTCTACC9200.034.12487456
GATCTCG16450.033.3460436
CCTGGGG24600.032.9602434
CGAATAC5200.032.50949569
TCGATAA9950.032.2461166
GCGCAAC652.3232773E-531.84570960
CGAGTAC13800.031.74988269
GTCAGCG10600.031.24484443