Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137873_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4866921 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA | 9303 | 0.19114754482351368 | No Hit |
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG | 7460 | 0.1532796607958091 | No Hit |
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT | 6931 | 0.14241036581444408 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 6437 | 0.1322602113327913 | TruSeq Adapter, Index 1 (96% over 28bp) |
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA | 5816 | 0.11950060418075412 | No Hit |
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC | 5668 | 0.11645966721054235 | No Hit |
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT | 5629 | 0.1156583392251487 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATCGTCA | 605 | 0.0 | 45.620605 | 10 |
TGGTCGT | 770 | 0.0 | 40.772575 | 51 |
GCTCGAT | 760 | 0.0 | 40.40075 | 64 |
TGCGCAA | 35 | 0.0013482721 | 39.428425 | 68 |
GTACCCG | 1430 | 0.0 | 37.39804 | 1 |
GCTCGTC | 860 | 0.0 | 36.505302 | 39 |
ATCTCGT | 1530 | 0.0 | 36.077923 | 37 |
GTCGTCT | 880 | 0.0 | 35.676373 | 53 |
TATGCCG | 1700 | 0.0 | 35.51421 | 43 |
ACGACGA | 1770 | 0.0 | 35.084255 | 65 |
GCATTAC | 3265 | 0.0 | 34.8697 | 69 |
GCGCTGG | 950 | 0.0 | 34.136715 | 47 |
GTCTACC | 920 | 0.0 | 34.124874 | 56 |
GATCTCG | 1645 | 0.0 | 33.34604 | 36 |
CCTGGGG | 2460 | 0.0 | 32.960243 | 4 |
CGAATAC | 520 | 0.0 | 32.509495 | 69 |
TCGATAA | 995 | 0.0 | 32.24611 | 66 |
GCGCAAC | 65 | 2.3232773E-5 | 31.845709 | 60 |
CGAGTAC | 1380 | 0.0 | 31.749882 | 69 |
GTCAGCG | 1060 | 0.0 | 31.244844 | 43 |