FastQCFastQC Report
Sat 18 Jun 2016
SRR3137872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244686
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258580.49303237600878297No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG69280.13209561068098263No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT56330.10740395135190171No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA52640.10036825846199372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG13050.038.120561
TACCTGG71700.030.8158262
TGCGACG7800.028.3090322
GTACCTG81550.027.7475471
GCTCGAT8250.027.59814664
ACCTGGG75850.027.4749873
TACCCGG17600.027.461762
TGGTCGT8550.027.43675251
ACCCGGG17100.026.4320813
ATCGTCA8500.025.97497410
ATGCGAC9000.024.9180821
GCTCGTC9650.024.66720839
CGACGAG8950.024.28583324
GTACTAG14600.022.7156751
GCGACGA9900.022.65258623
CCTGGGG49850.022.2835714
GTCGTCT10550.022.23547253
GTCAGCG10950.022.05331443
CTCGATA10350.021.99852265
CGTCATT11400.021.18500926
Produced by FastQC (version 0.11.5)