Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137872_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5244686 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 10088 | 0.19234707282762017 | RNA PCR Primer, Index 39 (96% over 30bp) |
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG | 7258 | 0.13838769375325807 | No Hit |
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT | 6090 | 0.11611753306108316 | No Hit |
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC | 5542 | 0.10566886177742577 | No Hit |
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA | 5491 | 0.1046964489389832 | No Hit |
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT | 5420 | 0.10334269773252393 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTACCCG | 1380 | 0.0 | 39.004032 | 1 |
TATGCCG | 2785 | 0.0 | 32.08372 | 43 |
ATCTCGT | 2745 | 0.0 | 31.169054 | 37 |
TCGTATG | 2880 | 0.0 | 30.905909 | 40 |
CTCGTAT | 2820 | 0.0 | 30.584768 | 39 |
TACCTGG | 7960 | 0.0 | 30.168947 | 2 |
TCTCGTA | 2845 | 0.0 | 29.830952 | 38 |
TGCCGTC | 3120 | 0.0 | 28.859983 | 45 |
TACCCGG | 1815 | 0.0 | 27.945036 | 2 |
CCGTCTT | 3175 | 0.0 | 27.925673 | 47 |
GTACCTG | 8870 | 0.0 | 27.851809 | 1 |
ACCTGGG | 8085 | 0.0 | 27.48336 | 3 |
ATGCCGT | 3305 | 0.0 | 27.244522 | 44 |
ACCCGGG | 1735 | 0.0 | 26.648287 | 3 |
ACGATCG | 325 | 0.0 | 26.53817 | 60 |
CGTATGC | 3425 | 0.0 | 26.08876 | 41 |
GCTCGAT | 1010 | 0.0 | 25.960108 | 64 |
GCCGTCT | 3590 | 0.0 | 24.88946 | 46 |
CGTCTTC | 3590 | 0.0 | 24.793596 | 48 |
TGGTCGT | 1075 | 0.0 | 24.390427 | 51 |