FastQCFastQC Report
Sat 18 Jun 2016
SRR3137872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244686
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG100880.19234707282762017RNA PCR Primer, Index 39 (96% over 30bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG72580.13838769375325807No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT60900.11611753306108316No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC55420.10566886177742577No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA54910.1046964489389832No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT54200.10334269773252393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG13800.039.0040321
TATGCCG27850.032.0837243
ATCTCGT27450.031.16905437
TCGTATG28800.030.90590940
CTCGTAT28200.030.58476839
TACCTGG79600.030.1689472
TCTCGTA28450.029.83095238
TGCCGTC31200.028.85998345
TACCCGG18150.027.9450362
CCGTCTT31750.027.92567347
GTACCTG88700.027.8518091
ACCTGGG80850.027.483363
ATGCCGT33050.027.24452244
ACCCGGG17350.026.6482873
ACGATCG3250.026.5381760
CGTATGC34250.026.0887641
GCTCGAT10100.025.96010864
GCCGTCT35900.024.8894646
CGTCTTC35900.024.79359648
TGGTCGT10750.024.39042751