FastQCFastQC Report
Sat 18 Jun 2016
SRR3137871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163909
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290840.698478280865408No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG56870.13657839304365202No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT46880.1125865142585969No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA44490.10684671543014028No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT42880.10298015638670298No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC42120.10115494839104314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG7250.032.8355924
GCTCGAT7300.031.6620364
TGCGACG8350.028.92313422
GTACTAG10100.028.7333741
GTACCCG10050.028.1887951
TGGTCGT8350.028.0937251
ATCGTCA8650.027.11937110
ACGAGTC8950.026.98382826
ATGCGAC9550.026.7345321
TACCTGG56350.026.653082
TCGTCTA8650.025.9229354
GCTCGTC9450.025.91902239
GTCGTCT8800.025.8730853
CTCGATA9450.024.45850265
GTACCTG64100.024.0384031
ACCTGGG56600.023.2276293
GTCAGCG10100.023.22599643
GGTCGTC15750.022.7792652
CGCACTA9700.022.76119658
CGTACGA4550.022.74556460
Produced by FastQC (version 0.11.5)