Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137871_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4163909 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 11757 | 0.28235487374964247 | RNA PCR Primer, Index 17 (96% over 28bp) |
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG | 5822 | 0.1398205388254162 | No Hit |
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT | 5257 | 0.12625155833136603 | No Hit |
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 5088 | 0.12219287213049085 | RNA PCR Primer, Index 17 (96% over 28bp) |
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA | 4669 | 0.11213021225968195 | No Hit |
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT | 4591 | 0.11025697247466262 | No Hit |
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC | 4532 | 0.10884003468855828 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 2985 | 0.0 | 32.59222 | 43 |
GTACCCG | 1040 | 0.0 | 31.519005 | 1 |
CTCGTAT | 2930 | 0.0 | 30.849129 | 39 |
TCGTATG | 3255 | 0.0 | 29.570755 | 40 |
GCTCGAT | 795 | 0.0 | 29.50876 | 64 |
TCTCGTA | 3065 | 0.0 | 29.490356 | 38 |
ATGCCGT | 3345 | 0.0 | 29.08454 | 44 |
TGCCGTC | 3365 | 0.0 | 28.911673 | 45 |
ATCTCGT | 3130 | 0.0 | 28.767717 | 37 |
CTCGATA | 855 | 0.0 | 27.841473 | 65 |
CCGTCTT | 3580 | 0.0 | 27.272053 | 47 |
GCCGTCT | 3655 | 0.0 | 26.712114 | 46 |
AATCTCG | 3420 | 0.0 | 26.328348 | 36 |
TGGTCGT | 865 | 0.0 | 26.323416 | 51 |
CGTATGC | 3720 | 0.0 | 26.245369 | 41 |
CGTCTTC | 3700 | 0.0 | 26.201069 | 48 |
GTATGCC | 3805 | 0.0 | 25.659073 | 42 |
TACCTGG | 5600 | 0.0 | 24.584824 | 2 |
TCGTCTA | 980 | 0.0 | 24.290552 | 54 |
GTCGTCT | 960 | 0.0 | 24.077864 | 53 |