FastQCFastQC Report
Sat 18 Jun 2016
SRR3137871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163909
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG117570.28235487374964247RNA PCR Primer, Index 17 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG58220.1398205388254162No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT52570.12625155833136603No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG50880.12219287213049085RNA PCR Primer, Index 17 (96% over 28bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA46690.11213021225968195No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT45910.11025697247466262No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC45320.10884003468855828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29850.032.5922243
GTACCCG10400.031.5190051
CTCGTAT29300.030.84912939
TCGTATG32550.029.57075540
GCTCGAT7950.029.5087664
TCTCGTA30650.029.49035638
ATGCCGT33450.029.0845444
TGCCGTC33650.028.91167345
ATCTCGT31300.028.76771737
CTCGATA8550.027.84147365
CCGTCTT35800.027.27205347
GCCGTCT36550.026.71211446
AATCTCG34200.026.32834836
TGGTCGT8650.026.32341651
CGTATGC37200.026.24536941
CGTCTTC37000.026.20106948
GTATGCC38050.025.65907342
TACCTGG56000.024.5848242
TCGTCTA9800.024.29055254
GTCGTCT9600.024.07786453