FastQCFastQC Report
Sat 18 Jun 2016
SRR3137870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781059
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72590.40756650958783514No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG29070.16321750149770445No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT23710.13312304645719206No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA22870.12840675126427592No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT19940.11195586446041371No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC18730.10516215352776073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT2900.041.63329364
GTACCCG4600.039.0647471
TGGTCGT3300.038.67750551
CTGGTCG3900.032.72712350
CTCGATA3850.032.25614565
TACCTGG25900.031.727672
GCTCGTC4250.030.8453539
GTCAGCG4300.030.48497443
GTCGTCT4100.030.28930553
GTCTACC4350.030.13457356
ACCTGGG23500.030.1008423
TCGTCTA4400.029.79213154
TACCCGG6600.028.2495042
ACCCGGG5400.028.1158713
CCTGGGG15150.027.7806424
GCGCTGG4900.027.45612347
ATCGTCA3100.026.70744910
CTGGGGG10650.026.560425
GTACCTG32550.025.9046861
CGCGCTA809.568321E-525.87356836
Produced by FastQC (version 0.11.5)