FastQCFastQC Report
Sat 18 Jun 2016
SRR3137870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781059
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG30830.17309926285429064No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT26080.1464297364657768No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG24390.13694099970860035TruSeq Adapter, Index 10 (96% over 28bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA22370.12559943269706395No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT20350.11425786568552755No Hit
GTACTAAGCAGTGGTATCAACGCAAAGCAGTGGTATCAACGCAGAGTACTAAGCAGTGGTATCAACGCAAAGCAG20290.1139209874574621No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC19830.1113382543756271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA3350.036.0447210
GTACCCG3750.034.9668161
ATCTCGT7750.033.8312537
GATCTCG7650.033.82252536
TATGCCG8700.032.91374243
GCTCGAT3550.032.0694764
TTTCGCC1650.031.36270333
CTCGTAT8600.030.88948639
TCGTATG9500.030.50521540
TACCTGG29250.030.4367032
TGCCGTC9650.030.03019745
CCGTCTT9800.029.2185247
CTCGATA3900.029.1914465
TCTCGTA8900.029.07298538
CGATCCG953.205514E-729.05258640
ATGCCGT10150.028.89157944
CGATAGC1800.028.74914663
TGGTCGT4400.028.22643351
ACCTGGG27050.028.0654073
CGTCTTC10250.027.93575748