Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137870_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1781059 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG | 3083 | 0.17309926285429064 | No Hit |
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT | 2608 | 0.1464297364657768 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 2439 | 0.13694099970860035 | TruSeq Adapter, Index 10 (96% over 28bp) |
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA | 2237 | 0.12559943269706395 | No Hit |
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT | 2035 | 0.11425786568552755 | No Hit |
GTACTAAGCAGTGGTATCAACGCAAAGCAGTGGTATCAACGCAGAGTACTAAGCAGTGGTATCAACGCAAAGCAG | 2029 | 0.1139209874574621 | No Hit |
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC | 1983 | 0.1113382543756271 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATCGTCA | 335 | 0.0 | 36.04472 | 10 |
GTACCCG | 375 | 0.0 | 34.966816 | 1 |
ATCTCGT | 775 | 0.0 | 33.83125 | 37 |
GATCTCG | 765 | 0.0 | 33.822525 | 36 |
TATGCCG | 870 | 0.0 | 32.913742 | 43 |
GCTCGAT | 355 | 0.0 | 32.06947 | 64 |
TTTCGCC | 165 | 0.0 | 31.362703 | 33 |
CTCGTAT | 860 | 0.0 | 30.889486 | 39 |
TCGTATG | 950 | 0.0 | 30.505215 | 40 |
TACCTGG | 2925 | 0.0 | 30.436703 | 2 |
TGCCGTC | 965 | 0.0 | 30.030197 | 45 |
CCGTCTT | 980 | 0.0 | 29.21852 | 47 |
CTCGATA | 390 | 0.0 | 29.19144 | 65 |
TCTCGTA | 890 | 0.0 | 29.072985 | 38 |
CGATCCG | 95 | 3.205514E-7 | 29.052586 | 40 |
ATGCCGT | 1015 | 0.0 | 28.891579 | 44 |
CGATAGC | 180 | 0.0 | 28.749146 | 63 |
TGGTCGT | 440 | 0.0 | 28.226433 | 51 |
ACCTGGG | 2705 | 0.0 | 28.065407 | 3 |
CGTCTTC | 1025 | 0.0 | 27.935757 | 48 |